Evaluation of Glycerol-Treated Liquid Pooled Serum with Special Reference to Stabilizing Effect on Lactate Dehydrogenase
1983 Volume 12 Issue 1 Pages 39-44
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1983 Volume 12 Issue 1 Pages 39-44
The addittion of ethylene glycol or glycerol to pooled human serum protects lactate dehydrogenase (LDH) activity during storage at-10°C or 4°C.At-20°Cethylene glycol caused a significant decreasein LDH activity during storage, the decrease depending on the proportion of added ethylene glycol.Reduced activity was accompanied by loss of the LDH3, LDH4 and LDH5 isoenzymes.ln contrast, glycerol addition to serum completely stabilizes LDH activity even at-20°C, and concomitant measurement of alkaline phosphatase, glucose and cholesterol showed no alteration in activity or concentrations.
Glycerol treated pooled serum would thus seem valuable for quality control for clinical use.
