Measurements of Oxygen and Carbon Dioxide Contents in the Blood with Gas Chromatography
1983 Volume 12 Issue 1 Pages 45-53
Details
1983 Volume 12 Issue 1 Pages 45-53
A gas chromatographic method for the quantitive determination of oxygen and carbon dioxide contents in the blood was investigated.A Shimazu gas chromatograph (GC -4CIT) and a Shimazu blood gas sampler (BGS-1A) were used for the analysis.
Oxygen and carbon dioxide were freed from blood with Van Slyke-Neil's reagent in BGS-1A and were directly transfered into gas chromatograph and digital integrator (Chromatopack-E1A) was used for calculating gas chromatographic peak area.
The reproducibility of the method was 0.8% (coefficient of variance) wlth the analysis of oxygen content and was 0.5% (C.V.) with the analysis of carbon dioxide content respe ctively.Agreement between gas chromatographic and Lex-02-Con values for oxygen content was excellent (r=0.980) and also excellent agreement was recognized for the analysis of carbon dloxlde content between gas chromatographic values and those obtalned from Van Slyke-Sendroy's nomogram (r=0.944).
ln this study, it wsa proved that this gas chrdmatographic method for the analysiis of oxygen and carbon dioxide contents in the blood was accurate enough to obtain satisfactory measurements and imposed relatively little skill and training in contrast to the conventional Van Slyke-Neil's manometric method.
