Multiple Forms of Glycylprolyl Dipeptidyl-Aminopeptidase in Serum and Tissues

Abstract

Multiple forms of glycylprolyl dipeptidyl-amhopeptidase (GP-DAP) were analyzed in human serum, liver, kidney, spleen, and lymphocytes by our methods based on electrophoresis with fluorometry using 7-glycyl-proline-4-methylcoumarinamide tosylate as substrate.Four peaks of the activity of GP-DAP were separated by the electrophoretic analysis.The main peak (peaklll) in normal human serum was found to coincide with that in the human spleen and lymphocytes.Peak ll and peak IV enzymes, which were predominantly observed in human sera from patients with hepatitis, coincided with the main peaks of crude and pure enzymes in the liver and kidney, respectively.