A Fluommetric Determination of Peroxidse Using o-Phenylenediamine as Hydrogen Donor

Abstract

A sensitive fluorometric determination of peroxidase activity using o-phenylenediamine as substrate is described.
Upon oxidation with peroxidase and hydrogen peroxide, o-phenylenediamine is converted to the2, 3-diaminophenazine which has an excitation maximum at 410nm and an emission maximum at 550 nm in neutral solution.Under the optimum conditbns, peroxidase was possible to determine in the range of 0.62 to 31.23μU/ml.