Abstract
A simple method for the determination of titer of total hemolytic complement activity is described.The principle of the method is based on the original CH 50 hemolysis method of Mayer.Modifications employed are;a reduced reaction volume, only a single colorimetric determination and addition of undiluted test serum to the reaction mixture.A table is constructed for easy calculation of the sample's titer.The assay is simple, rapid, economic and quantitative.The complement titers in sera measured by this new method correlate very well with those measured by the original method, r=0.998 (least squares regression), n=80.
