A Simple Sensitive Superoxide Dismutase (SOD) Assay and Its Serum levels among Various Pathological Conditions
1983 Volume 12 Issue 2 Pages 148-152
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1983 Volume 12 Issue 2 Pages 148-152
A simple and sensitive assay for serum superoxide dismutase (SOD) was deviced by using a xanthine-xanthine oxidase system in suitable pH condition and with Triton X-100.
Respective coefficients of variation for within-run and day-to-day precision of 3.8% and 8, 0% were obtained. The analytical recovery for sera supplemented with SOD was 96% and the normal range of serum SOD was 8.08±1.76 (mean±SD) U/ml.
Serum SOD activity were determined in adult patients of diabetes mellitus, hypertensive diseases, hepatitis, malignant tumors and lver-cirrhosis which seem to be attributable to oxigenic toxicity. Higher level of SOD was shown in several of these patients.
