A New Method for the Determination of β-Glucuronidase Activity in Enzyme Preparation Using Testosterone-Glucuronate and Androsterone-Glucuronate
1983 Volume 12 Issue 2 Pages 153-156
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1983 Volume 12 Issue 2 Pages 153-156
A new method for the determination of β-glucuronidase activity using steroidglucuronide as substrate has been described Principle of the reaction is as follows;steroid-glucuronide as substrate is hydrolyzed by the catalysis of β-glucuronidase and steroid released is determined by the enzymatic method using 3α-hydroxysteroid dehydrogenase or 3β, 17β, -hydroxysteroid dehydrogenase.
The specifisity of β-glucuronidase from E.coli and H.pomatia was compared with the conventional method using phenolphthalein glucuronide.
The results have shown that the measured activity of the enzyme was directly proportional to the enzyme concentrations and to the incubation time.The specificity of β-glucuronidase was the most for testosterone-glucuronate.
