Release of Several Enzymes from Rat Liver Microsomes by Various Solubilizing Treatments

Abstract

Rat linver microsomes were solubilized by treatments of detergents, hydrolyzing enzymes, sonication and freezing-thawing, and the effects of these solubilizing treatments On the release of four enzymes, NADH-cytochrome b5 reductase (Fp₁), NADPH-cytochrome c reductase (Fp₂), γ-glutamyltranspeptidase (γ-GTP) and glucose-6-phosphatase (G-6-Pase) from the microsomes were investigated.
Fp₁, Fp₂ and γ-GTP were releared easily by Triton X-100, a nonionic detergent.Fp₁ and Fp₂ were releared much more than γ-GTP by cholic acid, an anionic detergent.Trypsin or lipase digestion caused only the release of Fp₂ from the microsomes.ln the case of sonication treatment, the release of each enzyme with an increase in treatment time was not observed.These enzymes were released only a little by twenty times of freezing-thawing.
The present results suggest that Fp₂ might be most released from the membrane of damaged linver microsomes.