A New Automated Determination of Serum Apolipoprotein A-I by Immunoturbidimetry and its Clinical Applications
1984 Volume 13 Issue 1 Pages 31-39
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1984 Volume 13 Issue 1 Pages 31-39
It is well known that serum apolipoprotein A-I (apo A-I) is an important component of high density lipoproteins (HDL) and also plays some important physiologic roles. Although several technics for quantitating apo A-I have been reported, they are complicated technically and are not feasible for automation.
In this paper, we describe a new immunoturbidimetric assay for the determination of apo A-I by automated analysers.High precision and good correlation with the single radial immunodiffusion (SRID) method were obtained. The normal value of Apo A-I levels in male (n=82) and in female (n=82) were 122±24 mg/di (mean±S.D.) and 124±24 mg/di respectively. Apo A-I levels in patients with hepatitis, nephritisand old myocardial infarction were significantly decreased as compared with those of normals.
This method is useful and widely acceptable for the prediction of the risk of coronary heart disease in addition to HDL-cholesterol determination.
