Abstract
Reaction rate in enzyme catalysis, as the general chemical reactions, is a function of temperature, that is, increase of teperature causes increase of reaction rate (enzyme activity).in the enzyme reaction, generally, change of temperature of 1° Ccauses change of 7 to 10% of reaction rate. in this paper, we describe the relation of incubation temperature and enzyme activity using UV rate method which employs α-ketobutyric acid and NADH as substrate, and using Purified LDH isoenzymes, especially LDH1 and LDH2 which contain α-HBDH activity. As the results, 12% increase, only a little increase, of α-HBDH activity was found by the change of incubation temperature from 30° to 37°. We also found that the α-ketobutyric acid, as substrate, was used in low concentration to determine the α-HBDH activity in LDH1 and LDH2.
