A New Pseudoperoxidase Color Reaction
Determination of Hemoglobin Using 3-Methyl-2-Benzothiazolinone Hydrazone (MBTH) and Formaldehyde
1984 Volume 13 Issue 2 Pages 79-84
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1984 Volume 13 Issue 2 Pages 79-84
A sensitive and simple method fon color-producing detenmination of hemoglobin is described. The method is based on oxidative coupling of3-methyl-2-benzothiazolinone hydrazone (MBTH) with formaldehyde-ln the presence of hydnogen penoxide, hemoglobin shows a pseudopenoxidase effect and catalyzes the formation of a deep blue dye as the MBTH for maldehyde coupled, which has a broad absorption between 570 and 700nm with apeak at 635nm. ln the proposed method using the water-methanol (1: 1 by vol.) solvent system, blood components-denived interferences were elimirlated.The method was suited for the measurement of hemoglobin at concentnations of 1-20g/dl.The within-run pnecision (C.V.) was 2.03% with the recovery of 102.6%.A close correlation was found between this and the other methods.
