Abstract
A method for the determination of platinum in biological materials by using nickel as an internal standard was established.Ten μg of nickel was added to samples which contained platinum.Nickel spiked sample was digested with nitric acid and hydrogen peroxide. Acid digest was evaporated to dryness and the residue was dissolved in 0.5ml of IM nitric acid. The amount of platinum was measured byflameless atomic absorption spectrophotometer using background correction with deuterium Iamp, Nickel was measured by flame atomic absorption spectrophotometer. The amount of platinum measured was corrected by the amount of nickel measured.Limit of detection of platinum in the proposed method was 0.05 ppm and the calibration curve showed good linearity in the range from 0.1 to 2.0 ppm.
The method is advantageous in reducing sample size and making highly sensitive analysis of p-atinum possible.Correction for the loss of p-atinum resulted from bumping or pretreatment of sample is also easy.The method will be helpful in determining platinum in pharmacological and pharmacokinetic studies of various antineoplastic platinum complexes.
