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Volume 13, Issue 3
Displaying 1-6 of 6 articles from this issue
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KEIICHI BANDO, TOYOFUMI NAKANISHI, MASAAKI FUNAKI, KENICHI KISHIDA, CH ...1984 Volume 13 Issue 3 Pages 121-128
Published: August 25, 1984
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSA mass fragmentographic method for the simultaneus determination of phenobarbital, primidone and diphenylhydantoin levels in serum was developed.An on-column methylation technique was employed for the derivltizltion and 4-methylprimidone was used as the internal standard.The three drugs and the internal standard were well separated on the gas chromatogram.
Multiple ion detection with a chlracteristic ion of elch compound (m/z 232 for phenoblrbital, m/z 218 for primidone, m/z 280 for diphenlyhydantoin and m/z 260 for the internal standard) gave quantitltively satisflctory results.The overall recoverywas 96.3±8.3% (mean±S.D.) for phenobarbital, 102.6±8.8%for primidone and 98.0±6.7%for diphenylhydantoin. Correlation coefficients of the assay results with those of enzyme immunoassay method were 0.95 for phenoblrbital, 0.97 for primidone and 0.99 for diphenylhydantoin, respectively.
The present method is applicable for monitoring drug levels in human serum.View full abstractDownload PDF (811K) -
HIDEKI ISHIDA, MASAKO MAEDA, AKIO TSUJI1984 Volume 13 Issue 3 Pages 129-133
Published: August 25, 1984
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSHighly sensitive enzyme immunoassay using chemiluminescence reaction has been developed.Glucose oxidase was used as the labding enzyme and conjugated with anti-α-fetoprotein lgG.
Free and bound fractions after immune reaction, were separlted by insoiubilized antia-fetoprotein lgG.The enzyme activity was measured by chemiluminescence reaction.Hydro gen peroxide, generlted from glucose by glucose oxidlse reacts with isoluminol-microperoxidase system to produce chemiluminescence The luminescence intensity was linearly propotional to glucose oxidase activit.
The coupled reaction was used as a sensitive precise micro method for the determination of α-fetqprotein.The range of detection was 3-10ng/ml α-fetoprotein.View full abstractDownload PDF (446K) -
TATSUMORI TAKEDA, SHIGERU YOSHIDA, KAYOKO ODA, SHINGO HIROSE1984 Volume 13 Issue 3 Pages 134-139
Published: August 25, 1984
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSA highly sensitive fluorescence assay for total bile acid in serum using a flow injection system is described.
The applrltus consists of six-port valve, pretreatment column (2×20mm) packed serumout-25 (Sekisui chemical co.), immobilized enzyme column (3α-hydroxysteroid dehydrogenase, 3α-HSD, 2×30mm), injector, peristaltic pump and fluorescence detector.
This system enables to detect total bile acid from serum injection directly without sample pretreltment.The new device is especially useful for clinical studies where total bile acid has to be determined from serum.Moreover, the method was more sensitive and simple than conventional method.(cv=1.99%, n=10).View full abstractDownload PDF (555K) -
YASUHIRO NOMATA, AKIRA MATSUKAWA, KEN-ICHI HIRAOKA, IKUNOSUKE SAKURABA ...1984 Volume 13 Issue 3 Pages 140-147
Published: August 25, 1984
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSApoliooprotein A-II (apoA-II) was isolated from delipidated human serum high densit lipoprotein (HDL).HDL3 (density1.12-1.21) was preplred from fasting plasma of normolipidemic donors by sequential ultracentrifugation and fractionated on Sepharose 6B column chromatography.HDL3 wasdelipidated with ether-methanol (2: 1v/v) at tempera ture below -20°.Apolipoprotein A-II was then purified by DEAE-Sepharose CL 6B ion exchange column chromatography and Sephadex G-75 chromatogrlphy in the presence of 6M urea.The purity of the protein was ascertained by analytical polyacryamide gel electrophoresis in the presence of 6M urea or 0.1%SDS, amino acid analysis and immunochemical method.Purified apoA-II had molecular weight of 17,000 daltons by gel filtration on a Sephldex G-100 column and 18,000 by SDS polyacrylamide gel electrphorphoresis (without any reducing agent).The amino acid composition of purified apoA-II was consistent with the results previously reported.Antiserum against purified apoA-II was produced in rabbits and immunological properties of the antiserum were characterized.View full abstractDownload PDF (7851K) -
KAZUYUKI HIRANO, SHINOBU HAYAKAWA, TETSUO ADACHI, MAMORU SUGIURA1984 Volume 13 Issue 3 Pages 148-154
Published: August 25, 1984
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSA highly specific assay for the plasma kallikrein complex withα-macroglobulin has been developed.Plasma kallikrein-α-macroglobulin complex was quantitatively trapped by use of the polystyrene bead coated with the specific anti-α-macroglobulin antibody, and then the plasma kallikrein activity on the bead was determirned with Pro-Phe-Arg-4-methylcoumaryl-7-amide as a substrate.
The proposed method had a good precision. The detection range of plasma kallikrein-α2-macroglobulin complex was from 3 to 100 U/l. By the proposed method, the activity of plasma kallikrein in the complex in normal serum was found to be 44.8±3.7 U/l and the serum activity was significantly decreased in the patients with eiver cirrhosis.View full abstractDownload PDF (2692K) -
RYOICHI KIZU, MICHIKO HAMAKI, MITSUKO SHIMOZAWA, MOTOICHI MIYAZAKI1984 Volume 13 Issue 3 Pages 155-160
Published: August 25, 1984
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSA method for the determination of platinum in biological materials by using nickel as an internal standard was established.Ten μg of nickel was added to samples which contained platinum.Nickel spiked sample was digested with nitric acid and hydrogen peroxide. Acid digest was evaporated to dryness and the residue was dissolved in 0.5ml of IM nitric acid. The amount of platinum was measured byflameless atomic absorption spectrophotometer using background correction with deuterium Iamp, Nickel was measured by flame atomic absorption spectrophotometer. The amount of platinum measured was corrected by the amount of nickel measured.Limit of detection of platinum in the proposed method was 0.05 ppm and the calibration curve showed good linearity in the range from 0.1 to 2.0 ppm.
The method is advantageous in reducing sample size and making highly sensitive analysis of p-atinum possible.Correction for the loss of p-atinum resulted from bumping or pretreatment of sample is also easy.The method will be helpful in determining platinum in pharmacological and pharmacokinetic studies of various antineoplastic platinum complexes.View full abstractDownload PDF (708K)
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