Determination of Omithine Aminotmnsferase Activity by High-Performance Liquid Chromatogmphy Effect of KH₂PO₄ on Elution of Dihydroquimzolinium Compound

Abstract

Here we report an effect of monobasic potassium phosphate (KH₂PO₄) on the elution of dihydroquinazolinium compound from high-performance liquid chromatography, which is useful for the determination of ornithine aminotransferase activity.
A low concentration of KH₂PO₄ remarkably affected the elution time and the pattern of dihydroquinazolinium.
At the concentration between 3 and 5 mM, it was eluted as a sharp peak and separated from other peaks, otherwise it showed a broad peak or a peak which overlapped with other Deaks.