Abstract
A method for enzyme immunoassay of thyroid stimulating hormone (TSH) has been developed.TSH was conjugated with glucose oxidase (GOD) according to Nakane´s method, and the separation of free and bound fractions after immune reaction was achieved by using double antibody solid phase bead (DASP-bead).The working curve for TSH standards is satisfactory to recognize TSH concentration as 0.05μu/assay tube.
