臨床化学
Online ISSN : 2187-4077
Print ISSN : 0370-5633
ISSN-L : 0370-5633
高速液体クロマトグラフィーによるヒト赤血球アデニンおよびヒポキサンチン・ホスホリボシルトランスフェラーゼ活性の同時測定
佐久間 良三小島 司仁科 甫啓北村 元仕鎌谷 直之西岡 久寿樹
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1985 年 14 巻 4 号 p. 239-246

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A simple and rapid nonradiochemical method for the simultaneous determination of adenine phosphoribosyltransferase (APRT, EC 2. 4. 2. 7) and hypoxanthine phosphoribosyltransferase (HPRT, EC 2. 4. 2. 8) activities in human erythrocytes by high-performance liquid chromatography is described. APRT and HPRT activities were simultaneously assayed with adenine and hypoxanthine as substrates. adenosine-5'-monophosphate (AMP) and inosine-5'-monophosphate (IMP) produced by both enzyme reactions were converted to adenosine and inosine with alkaline phosphatase (EC 3. 1. 3. 1), respectively, and the adenosine and inosine separated from adenine and hypoxanthinelby a reversedphase silica column were determined by the absorbance at 270nm. The activities of APRT in erythrocytes of 36 healthy subjects were 0.42±0.17μmol/min/gHb and 2.00±0.39μmol/min/gHb (mean±2SD), respectively The method is suitable for screening erythrocytes for APRT and HPRT deficiencies.
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