抄録
We developed a new colorimetric method based on the oxidation and coloring reaction of leuco crystal violet (LCV) to determine hydrogen peroxide concentrations by the peroxidase-like activity of hemoglobin in the presence of hydrogen peroxide. The color intensity and hydrogen peroxide concentration were linear up to 1μM/ml. The minimum detectable concentration was 0.1μM/ml. The intra-assay coefficient of variation (CV) was 2.75% and the recovery rate was 104.0%.
