A Microquantity of Hemoglobin: Measurement and Application

抄録

The biochemistry, laboratory determination, and clinical or laboratory applications of very small quantities of hemoglobin are reviewed. No single assay method is suitable for the whole range of low concentrations, and for the forseeable future, as uniform standards are unlikely to be generally accepted, each laboratory must establish its own. Nevertheless, the development of an assay for very small amount of hemoglobin will be an important advance in many fields. There was a time when the chromogenic methods using benzidine and some part of its derivatives were used widely, because of their high sensitivity. However, since it was found that these chromogens were carcinogenic, many investigators have studied and reported alternative safety and sensitive methods. Here, based on these methods, we recommend the best available means of assessing the control of hemolytic anemia, screening for colorectal cancer, and monitoring blood storage.