Observation of the Varied Micro-Heterogeneous Forms of Human Urinary Kallikrein Individually Excreted in Some Diseases

Abstract

Excretion and heterogeneity due to varied carbohydrate structures of both active and latent types of human urinary kallikrein (HUK) in normal subjects and some patients were investigated.
Both active and latent types of HUK excretions were decreased in essential hypertension, chronic glomerulonephritis, chronic renal failure andnephrotic syndrome compared with normal subjects. In contrast, both types of HUK excretions were markedly increased in Bartter's syndrome. Analysis of sugar moieties of two types of HUKs in normal subjects and patients urine was carried out by the methods of seriallectin affinity chromatographies and crossed affino-immunoelectrophoresis. The analytical results derived from two different methods were almost agreed, especially in two diseases, i. e. essential hypertension and Bartter's syndrome, the alterations of chromatographic and electrophoretic patterns were observed and are supposed to correspond to glycosylation changes in each HUK.