Studies on Insulin and Anti-Insulin Antibody Levels in Sera of Insulin-Treated Patients

2) Evaluations of Anti-Insulin Antibody Index (Sebriakova), Antibody-Bound Insulin, and ¹²⁵I-Insulin Binding Ratio for the Determination of Anti-Insulin Antibodies in Sera

Abstract

Anti-insulin antibody index (Sebriakova), antibody-bound insulin, and 125I-insulin binding ratio were examined to determine anti-insulin antibody.
(1) Anti-insulin antibody index (sebriakova) gave good correlation with low-affinity insulinbinding capacity (Scatchard analysis)(r=0.89). It also correlated with antibody-bound insulin concentration (r=0.71).
(2) Antibody-bound insulin concentration gave good correlation with high-affinity insulinbinding capacity (Scatchard analysis)(r=0.73).
(3) ¹²⁵I-insulin binding ratio correlated poorly with low-or high-affinity insulin-binding capacity (Scatchard analysis), antibody-bound insulin, or anti-insulin antibody index (Sebriakova). But for ¹²⁵I-insulin binding ratios up to 50%, better correlation was obtained between ¹²⁵I-insulin binding ratio and antibody-bound insulin or anti-insulin antiboy index (Sebriakova).
These results suggest that anti-insulin antibody index (Sebriakova) and antibody-bound insulin are useful to ascertain low-affinity insulin binding capacity, and high-affinity insulinbinding capacity, respectively.