Abstract
We described a method estimating coexisting constituents which affect estimated value in the determination of various substances in body fluids. The principle of this method is based on the multiple regression analysis, by which the relationship between the concentrations of coexisting constituents and the difference between test method and comparative method was computed. Then, coexisting constituents contributing to the error were selected by the test of significance of partial coefficients. As an example of the application of this method, we studied the reaction of bromocresol green with serum protein fractions by cellulose acetate electrophoreiss.