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Volume 18, Issue 2
Displaying 1-7 of 7 articles from this issue
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YOSHIKAZU AOKI, KEIICHI MITSUTA, MASAHIRO KOHNO, HAJIME ITOH1989 Volume 18 Issue 2 Pages 35-41
Published: August 31, 1989
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSThe effects of oral administration of??-naphthylisothiocyanate (ANIT) on the microsomal membrane structure of rat liver were studied using the spin-labeling technique. Two different spin probes, 16-doxyl-stearic acid (16-DSA) and 5-doxyl-stearic acid (5-DSA), were used to assess the membrane fluidity. The rotational correlation time (τc) of 16-DSA decreased linearly, but temperature dependently, after ANIT administration; while it decreased sigmoidally in the control. Moreover, the levels of?Nc after ANIT administration were always shorter than those of the control between 22.5 and 37.5°C. On the other hand, the order parameter of 5-DSA incorporated in the ANiT-treated membrane did not differ distinctly from that of the control. These results suggest that ANIT or its metabolites injured the microstructure of the hydrophobic region rather than the surface region of the lipid bilayer in the membrane.View full abstractDownload PDF (798K) -
KENJI TOKUNAGA, OSAMU DOI, KOHJI SHISHINO, MITUHARU MURASE, NOZOMU TAK ...1989 Volume 18 Issue 2 Pages 42-48
Published: August 31, 1989
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSThe levels of superoxide dismutase (SOD), lipid peroxide (LPO) and ceruloplasmin in sera and synovial fluids were determined in rheumatid arthritis (RA), osteoarthritis (OA) and infectious arthritis (IA),
Total SOD, Cu, Zn-SOD activity and LPO level in synovial fluid from patients with RA and IA were found to be remakably higher than from OA, hower, Mn-SOD activity showed no difference.
The SOD activity in serum from RA was singnificantly lower than OA, but the LPO value Showed no difference.
Ceruloplasmin levels were increased in serum and synovial fluid from RA.
These results suggest that synovial fluid measurment may be useful in clinical evaluation of chronic inflammatory disorders.View full abstractDownload PDF (895K) -
OSAMU NOZAKI, TSUNEKO OHATA, YASUHIRO OHBA, HIROYUKI MORIYAMA, YOSHIO ...1989 Volume 18 Issue 2 Pages 49-54
Published: August 31, 1989
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSWe developed a method for measuring urinary 17 ketosteroids (17 KS) without prior hydrolysis of its conjugated forms. Both free and conjugated forms of 17 KS were successfully extracted by passing the samle through a BSA-ODS minicolumn (TSK), eluting with 80% methanol and drying. After the Zimmermann reaction, the Zimmermann chromogen was extracted with 1-butanol. Our proposed method gave rapid results for urinary 17 KS and showed good reproducibility (C. V.: 2.41-8.75%), recovery (about 100%) and correlation with the conventional method for determining 17 KS (r=0.881, a regression line, y=0.565x+4.52, n=25).View full abstractDownload PDF (746K) -
BAI ZHAOZHOU, SHAN HONGLING, LI CHIOUYUE1989 Volume 18 Issue 2 Pages 55-58
Published: August 31, 1989
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSWe detected a unique new pattern of amylase isonezyme is the urine of 6,708 Mongolians of ethnic Chinese descent. Four isozyme bands appeared on the electrophoretic gel, including a slow-moving variant of Amylase-1s and a dominant Amylase-2.We named this pattern “mixed type”. Pedigree study revealed that father has dominant Amylase-2 and mother Amylase-1s both traits being autosomal dominant. Typically, children of such a couple have equal chances of getting the five possible phenotypes (normal, dominant Amylase-2, Amylase-1s, and mixed) This is analagous to ABO blood type co-dominant inheritance.View full abstractDownload PDF (1693K) -
YUJI SUZUKI, SUSUMU EHARA1989 Volume 18 Issue 2 Pages 59-62
Published: August 31, 1989
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSWe described a method estimating coexisting constituents which affect estimated value in the determination of various substances in body fluids. The principle of this method is based on the multiple regression analysis, by which the relationship between the concentrations of coexisting constituents and the difference between test method and comparative method was computed. Then, coexisting constituents contributing to the error were selected by the test of significance of partial coefficients. As an example of the application of this method, we studied the reaction of bromocresol green with serum protein fractions by cellulose acetate electrophoreiss.View full abstractDownload PDF (407K) -
YOSHIKAZU AOKI, ZENSUKE OGAWA, HAJIME ITOH1989 Volume 18 Issue 2 Pages 63-67
Published: August 31, 1989
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSThe ketone body ratio in the blood, the ratio of acetoacetic acid to 3-hydroxybutyric acid, reflecting the energy charge in hepatic mitochondria, is an indicator of hepatic functional reserve in chronic liver injury. In the present study, changes in the ketone body ratio in the blood were determined in rats with acute and subacute choiestatic liver injury induced with α-naphthylisothiocyanate (ANIT). The ketone body ratio in the blood was significantly increased 12-60 hours after a single administration of ANIT. When the dose of ANIT was increased to 50-200 mg Per kg of body weight, the ratio was linearly increased. When ANIT was administered for 15 consecutive days, the ratio was increased to twice that of the control after 6 days of administration and remained the same until the 15th day. Thus, in acute and subacute cholestatic liver injury, the ketone body ratio showed a compensatory increase uniike the case for chronic liver injury.View full abstractDownload PDF (593K) -
ROBERT F. MORAN1989 Volume 18 Issue 2 Pages 68-75
Published: August 31, 1989
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSAs indicated at the outset of this report, the critical nature of the clinical requirements when blood gas testing is indicated, coupled with the labile nature of the sample and rapidly changing patient conditions makes it imperative that the analyst be aware of more than just the analysis it self Knowledge by the analyst, of the physiology of blood gas, the interpretation of basic blood gas results, the effects of the changing patient environment and the manner in which the sample is treated in a few short minutes from collection to reporting are equally important if one is to assure timely and clinically meaningful results.View full abstractDownload PDF (1049K)
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