Abstract
An automated method for the determination of pranoprofen, an anti-inflammatory drug, in human serum has been developed by means of column-switching high-performance liquid chromatography with ultraviolet detection (247 nm).A bovine serum albumin-coated ODS column (MK precolumn BSA-ODS) and a reversed phase column (TSK gel ODS-80TM) were used as the sample preparation column and analytical column, respectively. A twotimes diluted serum sample (20 μl) was applied onto the sample preparation column. The detection limit of pranoprofen is 0.05?Eg/ml serum. The recovery of pranoprofen added to serum (2.0-8.0 μg/ml) was approximately 100% with a standard deviation of 3% or less.
