Determination of Type IV Collgen Level in Serum with Monoclonal Antibodies and Its Application to Liver Diseases

Abstract

A sandwich ELISA system was developed using two monoclonal antibodies (JK- 199 and JK-132) against type IV collagen. The type IV collagen was determined in sera from liver diseases and healthy volunteers to assess relation of the protein level with clinical manifestations.
Type IV collagen concentration in sera of healthy volunteers (n=100) was 0-65 ng/ml, but was elevated up to 480 ng/ml in sera of patients with liver cirrhosis. Type IV collagen concentration in sera of patients with liver disease showed no correlation with the serum concentrations of GOT, GPT, Γ-GTP and PTV, indicators of liver diseases.
Either type IV collagen or PIIIP level was increased in sera from all the cases of hepatic carcinoma and/or liver cirrhosis compared to normal cases.