We describe the new test paper method for determining total serum bile acid (TBA). The test paper includes sodium oxamate, EDTA 2Na, 3α-HSD, NAD, diaphorase, NTB and buffer as a reaction mixture. Determination was performed employing blank test paper identical to our experimental paper except that it had no 3α-HSD
The reproducibility of this method was 10% (CV) around 10 μM and the recovery, between 80 and 111%. The coefficient of correlation to Mashige's method (Enzabile 2, Daiichi Pure Chemicals Co., Ltd.) was 0.993. Hemoglobin affected the present measurement system, whereas bilir- ubin, LDH, ascorbic acid and Intrafat did not.
TBA was estimated by this method in 23 normal subjects and 89 patients with various liver diseases. Mean normal value was 4.1 μM. TBA levels in patients with chronic active hepatitis and liver cirrhosis were more than 10μM even if their serum bilirubin levels were below 1.0mg/dl. Thus, we recommend this determination system for the massscreening of chronic liver diseases.
