Suppressed Dye-Binding of Bromcresol Purple with Serum Albumin by Bilirubin Conjugate, Ditaurobilirubin Sodium Salt

Abstract

We found that adding ditaurobilirubin (DTB) to normal adult serum decreased dye-binding with albumin in the bromcresol purple (BCP) method for serum albumin determination. The mixing of DTB and normal adult serum resulted in the appearance of delta bilirubin (Bd)-like material with the same retention time as authentic Bd on HPLC analysis. This bilirubin bound firmly to serum albumin. We consider that the peptide bond present in DTB reacts with serum albumin.