Japanese Journal of Clinical Chemistry

Significance of the Determination of More Accurate Cholesterol Contents in Foods in Relation to the Treatment of Atherosclerosis

Iwao Fukui

1990 Volume 19 Issue 4 Pages 333-345
Published: December 31, 1990
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.19.4_333

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Comparative Study of Interference of Mammalian Immunoglobulins on Human β-Endorphin Radioimmunoassay

Takuma Hashimoto, Machiko Nishibu, Fujitsugu Matsubara, Shunsuke Migit ...

1990 Volume 19 Issue 4 Pages 346-349
Published: December 31, 1990
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.19.4_346

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The relationship between P-endorphin and immunoglobulins was studied by examining the reaction of human and various animal IgGs to human β-endorphin radioimmunoassay (RIA).
The dilution curves of the highly purified IgGs of the human, rabbit, rat, guinea pig and goat samples, demonstrated 0.18%, 0.052%, 0.038%, 0.008%, 0.008%, and 0.007% displacement reactivity respectively, to the human P-endorphin standard curve, with good parallelism. These immunological parallels to human β-endorphin suggest that a part of the amino acid sequence of human and mammalian IgG is similar to that of human P-endorphin. Therefore, it can be speculated that this relationship between IgG and β-endorphin arises from the same origin in evolutionary terms, a possibility that needs further clarification.
Consequently, certain caution is warranted while interpreting the β-endorphin values obtained by the RIA using polyclonal antibody, particularly in a patient with high IgG levels, the cross-reaction makes the β-endorphin level spuriously high and to avoid IgG interference using a specific monoclonal antibody to synthetic β-endorphin is preferable to using polyclonal antibodies raised in animals.

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Action of Dilazep Dihydrochloride on Chemiluminescence from a Xanthine Oxidase/Acetaldehyde System

Yasunobu Yoshinaka, Toru Yokoyama, Masaki Nakamura

1990 Volume 19 Issue 4 Pages 350-353
Published: December 31, 1990
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.19.4_350

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Superoxide dismutase ISOD), catalase, hydroxyl radical (OH) scavengers and singlet oxygen (¹O₂) quenchers suppress the light signal from a xanthine oxidase/acetaldehyde system. Xanthine oxidase inhibitor allopurinol also suppresses the light signal from the system. Dilazep HCl (10^-5-10^-3M) suppresses the chemiluminescence in a concentration dependent manner without affecting the xanthine oxidase activity. These results suggest that dilazep exhibits a radical scavenger-like action.

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Determination of Etizolam in Human Serum or Plasma Using Automated Column-Switching High-Performance Liquid Chromatography

Kozo Hikida, Yoshimasa Inoue, Eiji Nouchi, Yosuke Ohkura

1990 Volume 19 Issue 4 Pages 354-359
Published: December 31, 1990
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.19.4_354

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An automated column-switching high-per formance liquid chromatographic method with ultraviolet detection is described for rapid and sensitive determination of the concentration of etizo- lam, an anti-anxiety drug, in human serum or plasma. Samples were subjected to column-switching high-performance liquid chromatography using a short precolumn packed with a bovine serum albumin-coated ODS for sample clean-up and a reversed phase column (TSK gel ODS-80TM) for analytical separation. The detection limit of etizolam is 0.7 ng/ml serum. The recovery of etizolam added to serum (9.8-39.4 ng/ml) was approximately 100% with a standard deviation of 3% or less.

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Determination of D-Amino Acid Oxidase in Urine

Takeshi Terazawa, Kiyoshi Okuda

1990 Volume 19 Issue 4 Pages 360-364
Published: December 31, 1990
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.19.4_360

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Studies on the Diazo Coupling Reaction in the Various Diazonium Salts for the Assay of Serum Bilirubin

Yuji Suzuki, Yoshikatsu Sakagishi

1990 Volume 19 Issue 4 Pages 365-373
Published: December 31, 1990
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.19.4_365

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Change for Aging of Bone and Liver Alkaline Phosphatase Isoenzymes in Serum of Healthy Subjects by Lectin Affinity Electrophoresis

Osamu Sugita, Reiko Wada, Tomomi Kuwana, Keiko Hirokawa, Minoru Yakata

1990 Volume 19 Issue 4 Pages 374-380
Published: December 31, 1990
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.19.4_374

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Studies on the Superoxide Generation of Neutrophils in the Patients with Liver Disease

Kenji Tokunaga, Koji Shishino, Mituharu Murase, Nozomu Takeuchi, Rikio ...

1990 Volume 19 Issue 4 Pages 381-388
Published: December 31, 1990
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.19.4_381

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A Comparative Study of the Clinical Usefulness of CA19-9-, CA50, Sialyl SSEA-1 and Dupan-2 for the Diagnosis of Pancreatic Cancer Used Alone or in Combination

Shigeyuki Kama, Hisao Oguchi, Masuo Tokoo, Takeshi Kobayashi, Haruhiko ...

1990 Volume 19 Issue 4 Pages 389-398
Published: December 31, 1990
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.19.4_389

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Serum levels of three tumor markers, CA50, sialyl SSEA-1 and Dupan-2 were determined in parallel with CA19-9 using serum samples from patients with various diseases including 170 patients with pancreatic cancer and up to 212 normal subjects. The objective was to determine whether any of those markers could compensate for the clinical drawbacks of CA19-9. None of these markers in a single determination was superior to CA19-9 in sensitivity and efficiency for the diagnosis of pancreatic cancer and the diagnostic efficiency of CA50 was almost comparable to that of CA19-9. Sialyl SSEA-1 was able to supplement CA19-9 to some extent in enhancing diagnostic specificity. No marker was superior to CA19-9 in the diagnosis of 17 patients with resectable pancreatic cancer. The combined use of CA19-9, sialyl SSEA-1 and Dupan-2 or of CA50, sialyl SSEA-1 and Dupan-2 increased the sensitivity but not the specificity. Thus, the diagnostic efficiency of these markers in combination was considered comparable to that of a single determination of CA19-9.

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Suppressed Dye-Binding of Bromcresol Purple with Serum Albumin by Bilirubin Conjugate, Ditaurobilirubin Sodium Salt

Hiroshi Ihara, Yutaka Aoki, Tsugutoshi Aoki, Mitsutaka Yoshida, Hiroom ...

1990 Volume 19 Issue 4 Pages 399-401
Published: December 31, 1990
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.19.4_399

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We found that adding ditaurobilirubin (DTB) to normal adult serum decreased dye-binding with albumin in the bromcresol purple (BCP) method for serum albumin determination. The mixing of DTB and normal adult serum resulted in the appearance of delta bilirubin (Bd)-like material with the same retention time as authentic Bd on HPLC analysis. This bilirubin bound firmly to serum albumin. We consider that the peptide bond present in DTB reacts with serum albumin.

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Reducing Effects of Serum Proteins on Organic Acid-Induced Inhibition of Serum Pseudocholinesterase Activity

Takafumi Sakoguchi, Kunio Kobayashi, Akira Matsuoka

1990 Volume 19 Issue 4 Pages 402-403
Published: December 31, 1990
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.19.4_402

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[title in Japanese]

1990 Volume 19 Issue 4 Pages 411-
Published: 1990
Released on J-STAGE: December 14, 2012

DOIhttps://doi.org/10.14921/jscc1971b.19.4_411

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