The Determination of Serum Triglyceride by the Enzymatic Hydrolysis

Abstract

A new enzymatic method is proposed for the determinatin of triglyceride in serum.
The principle of this method is as follows: Triglyceride is hydrolyzed into glycerol and free fatty acid by lipopr teinlipase (Psedomonas fluorescens) ycerol is transformed to pyruvate in the presence of glycerokinase, pyruvatekinase, ATP and.phosphoenol pyruvate.Being rea: ted with hydrazine, pyruvate forms hydrazone which shows the maximum absorbance at 450nm under the basic condition.
The amount of triglyceride is calculated following a certain formula from the value of optical density obtained at 450nm.
The values of serum triglyceride determined by the proposed method were well correlated with those by the chemical method and enzymatic (Boehringer UV) method after chemical saponification.The precision of the method was also satisfactory.
Since the enzymatic method is simple and specific for the measurment of triglyceride in comparison with the chemical method, the former is suitable for estimating triglyceride in a clinical laboratoy.