Eletrophoretic Examination of Human Serum Amylase lsozymes

Abstract

The lectrophtoercehtniiwcqi uteah s implteh ilna yepro lyacrylgaemlpir doevided a good resolution of serum amylase isozymes, with up to 9 being observed.From ther esulotfst hee lectrophomreobticl isttyu dieofss erum, pancreataincd salivaarmyy lase isozymes, we considered that the amylase isozymes in human serum were amixture of pancreatic and salivary amylase. In patients with acute pancreatitis, the activities of the amylase isozymes of pancreatic origin were increased.On the contrary, in patients with pancreatectomy, these isozymes disappeared. In patients with mumpus, the activities of amylase isozymes of salivary origin were increased.Only these amylase isozymes of salivary origin were observed in patients with pancreatectomy. From these observations, it is clear that amylase isozymes in human serum were of pancreatic and salivary origin.In normal sublects, 2 major bands of amylase isozymes and 2 to 4 minor bands were observed. All these amylase isozymes showed the same electrophoretic mobility as pancreatic or salivary amylase isozymes.