1991 Volume 20 Issue 3 Pages 156-161
Experiments employing dichlorofluorescin-diacetate as a label for flow-cytometry were undertaken in order to detect the hydrogen peroxide generated by neutrophils and myocardial cells. Following treatment with anoxia and reoxygenation, intracellular dichloro-fluorescin was found to have been oxidized to a highly fluorescent substance by the neutrophils. In contrast, the fluorescence intensity of the myocardial cells following treatment was only slightly increased. The effects of anoxia and reoxygenation on a mixture of myocardial cells and neutophils were also studied. The dichlorofluorescin in the myocardial cells was oxidized by hydrogen peroxide generated by the neutrophils. Pyridine, an inhibitor of cytochrome b558, inhibited the oxidation of dichlorofluorescin in myocardial cells.
