A Chemiluminescence Method for the Determination of D-3-Hydroxybutyric Acid in Blood Using 3-Hydroxybutyric Dehydrogenase/Isoluminol·Micro Peroxidase

Abstract

We hereby would like to describe a highly sensitive chemiluminescence method used for the determination of 3-hydroxybutylate in serum using 3-hydroxybutyric dehydrogenase/isoluminol· micro peroxidase (3-HBDH/isoluminol·micro peroxidase) a procedure for the preparation of protein-free supernatant (Somogyi method).
The method requires only 25μl of pricked ear blood or venous blood and could detect 3-hydroxybutylate ranging from 5-250 pmol/test tube.
The difference values of 3-hydroxybutylate contained in blood have a range of 12-103 (mean 34) μmol/l.
This method is suitable for the efficient screening of the diagnosis and treatment of diabetes.