ELISA(Enzyme-Linked Immunosorbent Assay)法による新生児血液ろ紙中のグリココール酸の定量

池田 ひろみ; 大熊 博; 前田 昌子; 辻 章夫; 松井 陽; 神戸川 明

doi:10.14921/jscc1971b.21.2_86

ELISA (Enzyme-Linked Immunosorbent Assay) for Glycocholic Acid in Neonatal Blood Spotted on Filter Paper

Hiromi Ikeda, Hiroshi Ohkuma, Masako Maeda, Akio Tsuji, Akira Matsui, Akira Kanbegawa

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Keywords: enzyme-linked immunosorbent assay, glycocholic acid, congenital biliary atresia, mass screening, horseradish peroxidase

JOURNAL FREE ACCESS

1992 Volume 21 Issue 2 Pages 86-91

DOI https://doi.org/10.14921/jscc1971b.21.2_86

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Abstract

We have developed an enzyme-linked immunosorbent assay for glycocholic acid in dried blood spotted on a filter paper.
The linear range of this assay was 1 to 32 nmol/ml and the coefficient of variation of within and between the assay range was 2.6-8.9% and 3.4-8.2%, respectively.
The average concentration of the glycocholic acid value in normal newborn babies was 8.25 nmol/ml, and that in babies with congenital biliary atresia (CBA) was 49.26nmol/ml (n=24).
This method will be applicable in mass screening for CBA.

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