Precision Assay of Bone-Type Alkaline Phosphatase

Abstract

We investigated the determination of bone-type alkaline phosphatase (ALP) with densitometric scan using polyacrylamide disc gel electrophoresis (PAGE). The CV obtained in precision study was less 2% for within-run assay, and the linearity extended down to 20 mU/ml. The measured values with the PAGE method were correlated well with cellulose acetate membrane (CA) electrophoresis method, but it was appeared that the PAGE method with densitometric scan was more correctly detected bone-type ALP than CA method because of good separating liver-and bone-type ALPs. Moreover, the normal reference values of bonetype ALP (mean±2SD), obtained 68 healthy adults was 53±36.6 mU/ml. This result was similar to that of WGA affinity electrophoresis as described earlier. We conclude that the precision determination of bone-type ALP is useful metabolic bone marker for diagnosis of bone disease.