Separation and Identification of Human Serum Protein with Capillary Electrophoresis System
1994 Volume 23 Issue 1 Pages 32-37
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1994 Volume 23 Issue 1 Pages 32-37
We have demonstrated the separation of human serum protein with capillary electrophoresis (CE) system. A untreated fused silica capillary, 20μm (I. D.) ×20cm, was assembled in the CE cartridge format. On-line detection of CE system was at 200nm. The capillary temperature was maintained at 23°Cduring electrophoresis. Human serum samples were diluted 11-fold in 20mM PBS (pH7.0). Diluted samples of about 0.2nl were introduced by pressure injection for 10 s. The analysis buffer was used 150mM borate buffer (pH 10.0) and electrophoresis was performed at 10 KV for 6.5 min.
Human serum sample was separated five fractions by CE and was identical with those of cellulose acetate membrane electrophoresis. We present our method for separation of serum proteins into 10 peaks. These protein peaks were detected immunoglobulins, complement C3, transferrin, α2-macroglobulin, haptoglobin, α1-antitrypsin, albumin, preablumin, respectively, according to fast migration time with CE. Moreover, capillary electrophoretic patterns in various patient sera were in complete agreement with the results shown by cellulose acetate membrane electrophoresis.
From these results, it is suggested that CE system is useful techonology for clinical diagnosis and suitable tool for routine work in the future.
