Abstract
An improved colorimetric method for the determination of phosphatidylcholine hydroperoxide is described. Phosphatidylcholine hydroperoxide was allowed to react with potassium iodide in the presence of acetylchloride at room temperature, and the liberated iodine was measured colorimetrically at 550 nm after the addition of starch in 10% aqueous acetic acid. A linear response was observed between 5 and 40 nmol.