1994 Volume 23 Issue 2 Pages 182-187
A highly sensitive and selective high-performance liquid chromatography (HPLC) method with ultraviolet detection was developed using column- switching system for the simultaneousdetermination of hypoxanthine (HX) and xanthine (XA) in human plasma and urine. The selective separation of this method is based on the combination of the column-switching technique and two different separation modes of chromatography (reversed- phase and cation- exchange chromatography).
By this method the successive simultaneous analysis of the two in any sample could be performed by the same HPLC conditions in a 35- min cycle. The lower limits of detection for HX and XA were both approximately 0.8 pmol per injection. The recoveries were 97- 103% and the coefficients of variation were less than 3%.
