Enzymatic Determination of the Chloride Ion Concentration Using 3-Ketobutylidene β-2-Chloro-4-Nitrophenylmaltopentaoside (3KB-βCNPG5)

Abstract

3-Ketobutylidene β-2-chloro-4-nitrophenylmaltopentaoside (3KB-βCNPG5) was used for the determination of the chloride ion concentration in serum and urine. This enzymatic assay for the chloride ion, which is based on the determination of α-amylase using 3KB-β CNPG5, has a wide dynamic range (0-400mmol/l) and is less affected by other endogenous anions in biological fluids than other methods. This method is highly sensitive and stable for determing the chloride-ion concentration and can be applied to undiluted samples of sera and urine.