Enzymatic Determination of the Chloride Ion Concentration Using 3-Ketobutylidene β-2-Chloro-4-Nitrophenylmaltopentaoside (3KB-βCNPG5)

Keiichi Majima; Shinichi Teshima; Katsuhiko Mizuguchi; Toshiro Kikuchi; Yoshihisa Kawamura

doi:10.14921/jscc1971b.25.4_223

Keiichi Majima, Shinichi Teshima, Katsuhiko Mizuguchi, Toshiro Kikuchi, Yoshihisa Kawamura

著者情報

キーワード: chloride, 3-ketobutylidene β-2-chloro-4-nitrophenylmaltopentaoside (3KB-βCNPG5), enzymatic determination method

ジャーナルフリー

1996 年 25 巻 4 号 p. 223-228

DOI https://doi.org/10.14921/jscc1971b.25.4_223

詳細

抄録

3-Ketobutylidene β-2-chloro-4-nitrophenylmaltopentaoside (3KB-βCNPG5) was used for the determination of the chloride ion concentration in serum and urine. This enzymatic assay for the chloride ion, which is based on the determination of α-amylase using 3KB-β CNPG5, has a wide dynamic range (0-400mmol/l) and is less affected by other endogenous anions in biological fluids than other methods. This method is highly sensitive and stable for determing the chloride-ion concentration and can be applied to undiluted samples of sera and urine.

責任著者(Corresponding author)

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