抄録
A one-tube method for measuring both the serum iron concentration and iron-binding capacity is described. Iron is released from transferrin in an acid solution and combined to TPTZ. The color intensity is proportional to the serum iron concentration.
An alkaline buffered solution is added to the reaction mixture to make the contents alkaline, dissociate the iron from TPTZ-iron complex, and permit to recombine with transferrin. Subseqently, a known excess of iron is added to the test tube to saturate the iron-binding protein. The surplus unbound iron in solution is determined by complexing it with SBP (Sodium Bathophenanthroline Sulfonic Acid), and this color formed iron-SBP chelate is very stable above pH 9.0. The unsaturated iron-binding capacity of the serum specimens is calculated by the determination of the difference between the quantity of iron added to the test solution and the amount of iron that complexes with SBP.
