An Approach to Clinical Test of Mini-Column Chromatoraphy with DEAE-Sephadex A-50 I. Determination of IgG in Human Serum

Abstract

Determination of serum IgG was studied and a new method was devised. After application of 50μl of serum to a mini-column with Sephadex A-50, 0nly lgG was rapidly eluted in the non-adsorbed fraction. This lgG fraction was determined as the amount of protein by biuret method. By the use of this mini-column chromatography, IgG may be rapidly fractionated with a constant volume of the eluate despite the use of various serum samples. By the use of standard lgG, 98.5% of the applied sample was recovered, with Iittle influence by other proteins in serum. The results obtained by this method were found to exhibit an excellent correlation with those obtained by the well known SRID method. This method thus appears to be useful in daily clinical test.