Abstract
A sensitive and rapid spectrophotometric method for the determination of elastase (EC3.4. 4.7) by its elastoproteolytic activity with insoluble elastin as a substrate, is described.
Elastase activity was not found in human serum and elastase inhitor capacity in serum could be determined by this method. It was possible to determine elastase activity in pancreatic Juice of dogs by this method.
These results suggest that this method will be a useful test for detecting pancreatic dysfunction.
