Separative assay method of GOT isozyme using cellulose acetate plate electrophoresis

Abstract

Serum GOT activity is elevated in hepatitis and myocardial infarction patient. Boyd1) showed that by means of starch gel electrophoresis there were two kinds of GOT isozyme in the sera from hepatitis patient and each of them was established as GOT-m and GOT-s from human liver. However the starch gel is not suitable for the routine method because of time wasting and incomplete quantative estimation. This paper reports our studies to separate and determine the GOT isozyme in sera.
1. The determination of each GOT isozyme activity was done after the separation using the method of plate electrophoresis.
2. It is possible to obtain the separation and determination of each isozyme activity between 5 and 200Karmen unit/ml within one hour and to estimate 32 samples (sera) at the same time.
3. The distributions of the isozymes invarious organs were reinvestigated using this method.
4. GOT isozyme activities were separated and determined in sera of hepatitis and myocardial infarction by using this method.