臨床化学
Online ISSN : 2187-4077
Print ISSN : 0370-5633
ISSN-L : 0370-5633
水解に及ぼすNa2SO4効果
フォルクマー グレーフ古屋 悦子西風 脩
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ジャーナル フリー

1977 年 5 巻 2 号 p. 182-188

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抄録
The rate of hydrolysis of steroid glucuronides (poregnanediol-, and 17-hydroxycorticosteroid- glucuronides) is increased by an addition of sodium sulfate to the incubation medium, when usinng β-glucuronidase preparations from bovine liver.
But, sodium sulfate inhibits the enzyme hydrolysis of pregnanediol-, and estriol-glucuronides with preparations from Helix pomatia and from E. coli.
The rate of hdrolysis of nonsteroid glucuronides (P-nitrophenyl-and phenolphthaleinglucuronides) is increased by sodium sulfate when using Preparations from bovine liver and Helix pomatia, it is inhibited with preparations from E. coli.
The preparation from Helix pomatia consists of four glucuronidase fractions, none of which hydrolyzes steroid glucuronides specifically.
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