臨床化学
Online ISSN : 2187-4077
Print ISSN : 0370-5633
ISSN-L : 0370-5633
《技術》抽出操作を省いた血清テストステロンの直接ラジオイムノアッセイ法の検討
立石 カヨ子加藤 天美林 長蔵
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1978 年 6 巻 3 号 p. 218-221

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A direct method for the radioimmunoassay of male serum testosterone is described which does not require extraction by organic solvents. Interference from sex hormone binding proteins is removed by adding a gross excess of estradiol to each sample. Separation of bound and free 3H-testosterone is by means of serum dextran coated charcoal. Data indicating the accuracy and reproducibility of this method are presented and the possibility of one-step radioimmunoassay of serum testosterone is discussed.
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