Abstract
On the electrophoretic enzymogram of serum lactate dehydrogenase isoenzymes, an extra band was found cathodaly to LDH-5 (M4).
This extra band was identified as alcohol dehydrogenase (ADH) activity by the following evidence; the extra band was still observed even when lactate was omitted in the LDH reaction system, its electrophoretic mobility was corresponded to that of humau liver ADH isoenzyme, the activity of this band was increased by adding of ethanol to the reaction mixture of LDH isoenzyme and was inhibited by Pyrazole.
The mechanism responsible for the appearance of ADH activity is that alcohols in the specimen are coupled with NAD-PMS-tetrazolium salt system which is involved in the LDH reaction mixture, to from formazan. The ADH band was found in the serum of patients with severe liver cell necrosis due to acute hepatitis, acute heart failure and bile duct stone.
