Abstract
A Simple analytical method for kallikrein (E. C. 3, 4, 21, 8) by electrophoresis on a cellulose acetate membrane was established. Detection of the components which could hydrolyze BAEE, such as kallikrein, trypsin, etc., was carried out on the membrane by a reaction with BAEE and then by a modified formazan stain.
Various preparations of hog pancreatic kallikrein of different purities were used. Kallikrein migrated towards the anode and there were two or more active components in these kallikrein preparations. Clear separation from trypsin was possible by this system.
The procedures are simple and this method is suitable for the detection of kallikrein even in a crude preparation.
