Abstract
A method for determination of Plasma dehydroepiandrosterone sulfate using 3 β-hydroxysteroid oxidase is described. The method includes lipides removal and protein precipitation by adding a mixture of chloroform and methanol (2: 1, v/v), and solvolysis after extraction with ethylacetate under pH 1 with NaCl saturation, and fluorometric determination using 3 β-hydroxy-steroid oxidase, peroxidase and homovanillic acid by the method of G. G. Guilbault et al.
Precision of this method was 5.7% of CV and recovery was 98.2%. These results were satisfactory for determination of plasma dehydroepiandrosterone sulfate in clinical test. An advantage of the method is that the determination can be performed without use of radioactive compound.
