1979 Volume 7 Issue 4 Pages 411-418
In a cause of studies on constituents in humann serum, the spots of the corresponding cholesterol (FC) and cholesterol ester (CE) in thin-layer chromatography for the quantitative analysis by dual wave-length thin-layer chromatography-densitometry have been studied.
The human sera were treated to separate into individual components by means of column chromatography, thin layer chromatography and preparative gas chromatography. The structure determination of components were performed on the ground of chemical and spectraum evidence.
The chemical treatment was shown in the text. The reduction products of cholesterol ester (CE) with lithium alumium hydride (LAH) were easily analyzed by gas chromatography.
