Enzymatic Determination of Serum Uric Acid

Using Uricase and NADH-Peroxidase

Abstract

Enzymatic determination of serum uric acid using uricase (EC. 1. 7. 3. 3) and NADH-peroxidase (EC. 1. 11. 1. 1.) is described.
The principle of this method is as follows: Uric acid is degradated by uricase to produce allantoin and H₂O₂, and the H₂O₂ produced is coupled with NADH-peroxidase. The change in absorbance at 340 nm is measured. Standard curve was linear up to, at least, 20mg/dl in concentration. Correlation between present method and UV method (assay at 291 nm) was compared, and the correlation coefficent value was 0.998 and regression line was y=1.00x-0.08 (y: present method).