Application of Electron Spin Resonance (ESR) to Determination of Total Protein in Cerebrospinal Fluid

Abstract

Determination of total protein in cerebrospinal fluid using electron spin resonance is described. As signal intensity of ESR is decreased by addition of protein to spin labeled compound, a good standard curve is obtained using human serum (or control serum), which is similar to cerebrospinal fluid in composition.
Sample volume used in this method is only 100μl, and the comparison of values with Esbach method has also been performed, but the values by present method was higher than that of Esbach method in like result of high values by biuret method.